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ab 528427 en1 4g11  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank ab 528427 en1 4g11
    Ab 528427 En1 4g11, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ab 528427 en1 4g11/product/Developmental Studies Hybridoma Bank
    Average 94 stars, based on 74 article reviews
    ab 528427 en1 4g11 - by Bioz Stars, 2026-03
    94/100 stars

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    (A) Schematic depiction of the protocol for differentiating mDA neurons, illustrating the application of pharmacological compounds and growth factors. Representative immunocytochemical images and quantification from both original protocol and new protocol showing (B, E) efficacies of midbrain floorplate patterning at DIV11, showing co-localizations of <t>LMX1+/EN1+</t> and SOX6+/OTX2+; and EN1+/SOX6+/TH+ neurons at DIV 16 (C, F) and 28 (D, G). White scale bars = 50, 100 µm as indicated (n = 3 independent experiments, error bars are SEM, ns = not significant; * = p<0.05; ** = p<0.01; *** = p<0.001; **** = p<0.0001). (H) Calcium imaging recordings of spontaneous and KCl-induced activities on DIV 28 and 42, presenting the total number of calcium events per neuron recorded, and the percentages of cells responding to KCl-induced depolarization. Metrics of activities, including amplitude, rise time, and fall time of calcium signals, are depicted in the subsequent bar plots.
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    (A) Schematic depiction of the protocol for differentiating mDA neurons, illustrating the application of pharmacological compounds and growth factors. Representative immunocytochemical images and quantification from both original protocol and new protocol showing (B, E) efficacies of midbrain floorplate patterning at DIV11, showing co-localizations of LMX1+/EN1+ and SOX6+/OTX2+; and EN1+/SOX6+/TH+ neurons at DIV 16 (C, F) and 28 (D, G). White scale bars = 50, 100 µm as indicated (n = 3 independent experiments, error bars are SEM, ns = not significant; * = p<0.05; ** = p<0.01; *** = p<0.001; **** = p<0.0001). (H) Calcium imaging recordings of spontaneous and KCl-induced activities on DIV 28 and 42, presenting the total number of calcium events per neuron recorded, and the percentages of cells responding to KCl-induced depolarization. Metrics of activities, including amplitude, rise time, and fall time of calcium signals, are depicted in the subsequent bar plots.

    Journal: bioRxiv

    Article Title: Development of a protocol utilizing single-cell analysis for the differentiation of human iPSCs into SOX6+ midbrain dopaminergic neurons

    doi: 10.1101/2025.06.18.660098

    Figure Lengend Snippet: (A) Schematic depiction of the protocol for differentiating mDA neurons, illustrating the application of pharmacological compounds and growth factors. Representative immunocytochemical images and quantification from both original protocol and new protocol showing (B, E) efficacies of midbrain floorplate patterning at DIV11, showing co-localizations of LMX1+/EN1+ and SOX6+/OTX2+; and EN1+/SOX6+/TH+ neurons at DIV 16 (C, F) and 28 (D, G). White scale bars = 50, 100 µm as indicated (n = 3 independent experiments, error bars are SEM, ns = not significant; * = p<0.05; ** = p<0.01; *** = p<0.001; **** = p<0.0001). (H) Calcium imaging recordings of spontaneous and KCl-induced activities on DIV 28 and 42, presenting the total number of calcium events per neuron recorded, and the percentages of cells responding to KCl-induced depolarization. Metrics of activities, including amplitude, rise time, and fall time of calcium signals, are depicted in the subsequent bar plots.

    Article Snippet: The primary antibodies were used as follows: CORIN (rat, 1:1,000, R&D Systems, MAB2209), DCX (goat, 1:500, SantaCruz, sc-8066), EN1 (mouse 1:50, DSHB, 4G11), FOXA2 (goat, 1:500, R&D Systems, AF2400), GIRK2 (rabbit, 1:400, Alomone, APC006), LMX1A (rabbit, 1:4,000, Millipore, AB10533), LMO3 (goat, 1:200, SantaCruz, sc-82647), MAP2 (mouse 1:1,000, Sigma, M4403), NURR1 (rabbit, 1:500, SantaCruz, sc-990), OTX2 (goat, 1:1,000, R&D Systems, AF1979), TH (rabbit, 1:1,000, Millipore, AB152), TH (mouse, 1:500, ImmunoStar, 22941), and TH (sheep, 1:500, Novus, NB300).

    Techniques: Imaging

    Journal: iScience

    Article Title: Molecular diversity and migration of GABAergic neurons in the developing ventral midbrain

    doi: 10.1016/j.isci.2024.111239

    Figure Lengend Snippet:

    Article Snippet: The following primary antibodies were used: rabbit anti-TH 1:500 (Millipore, Cat# AB152, RRID: AB_390204 ), sheep anti-TH 1:500 (Millipore, Cat# AB1542, RRID: AB_90755 ), mouse anti-TH 1:500 (Millipore, Cat# MAB318, RRID: AB_2201528 ), chicken anti-TH (Aves labs, Cat#TYH, RRID: AB_10013440 ), rabbit anti-Six3 1:1000 (Rockland, Cat# 600-401-A26, RRID: AB_11180063 ), rabbit anti-Pax5 1:500 (Abcam, Cat# ab109443; RRID: AB_10862070 ), goat anti-Sox21 1:200 (R&D Systems, Cat# AF3538, RRID: AB_2195947 ), rat anti-CTIP2 1:1000 (Abcam, Cat# AB18465, RRID: AB_2064130 ), rabbit anti-Pou6f2 1:500 (Sigma-Aldrich, Cat# HPA008699, RRID: AB_1079664 ), goat anti-Otx2 1:500 (R&D Systems, Cat# Af1979, RRID: AB_2157172 ), mouse anti-En1 1:40 (DSHB, Cat#4G11, RRID: AB_528219 ), mouse anti-Nkx2.2 1:10 (DSHB, Cat#74.5A5, RRID: AB_531794 ), mouse anti-Pax7 1:50 (DSHB, Cat#pax7, RRID: AB_528428 ), goat anti-tdTomato 1:500 (SICGEN, Cat#AB8181, RRID: AB_2722750 ), rabbit anti-RFP 1:500 (Rockland, Cat#600-401-379, RRID: AB_2209751 ), rabbit anti-GFP 1:2000 (Life technologies, Cat#A11122, RRID: AB_221569 ).

    Techniques: Recombinant, Fluorsave, Gel Extraction, Generated, Software, Gene Expression